Sunday, May 8, 2022 12:30 PM – 1:15 PM ET Exhibit Hall, Workshop Room 1
Exhibitor Workshop: FlowJo, LLC
Vidi Vici Idolum: A Guide to Conquering High-dimension Data from Clean-up to Trajectory Analysis
- Jack Panopoulos, Ph. D., Application Scientist
A diverse repertoire of algorithmic tools is needed to identify novel phenotypes, functional states, and disease correlates from high-dimensional data. In this tutorial we will review the primary classes of algorithm applicable to cytometric data, and then demonstrate a high-dimensional workflow using an exemplar from each class. We will then push the traditional bounds via a trajectory analysis algorithm, TrackSOM. The nature of the cytometric data tends to confine analysis to a ‘snapshot’ of cellular dynamics. Trajectory analysis follows population dynamics over multiple time points, adding a layer of functional analysis that was previously difficult to ascertain.