Sunday, May 8, 2022 12:30 PM – 1:15 PM ET Exhibit Hall, Workshop Room 3
Exhibitor Workshop: Emulate
Modeling Inflammatory Immune Cell Recruitment and Response with the Colon Intestine-Chip
- Christopher Carman, Ph.D., Director of Immunology
Recruitment of circulating immune cells into tissues is a key driver of complex human diseases, such as inflammatory bowel disease (IBD). To model IBD, we perfused peripheral blood mononuclear cells (PBMCs) through the Emulate Colon Intestine-Chip’s vascular channel. In the presence of priming cytokines/chemokines, we observed selective migration of ‘gut-specific’ (α4β7+) PBMCs into the epithelial channel where they secreted IBD cytokines (e.g. INFγ, IL1 β, IL18) and induced barrier disruption. These events were abrogated by clinically relevant α4β7+ targeting drugs, suggesting the model may provide a more human-relevant platform for IBD drug development.