Sunday, May 8, 2022 1:45 PM – 2:30 PM ET Exhibit Hall, Workshop Room 1
Exhibitor Workshop: BioLegend
Cutting-Edge Technology for Deep Characterization of Viral and Self-Antigen Peptide-Induced Cellular Immune Responses
- Rebecca Nickle, Ph.D., Technical Applications Scientist
Biological processes are complex, and to understand them in a holistic manner, researchers need to use multiple tools and innovative approaches to capture that complexity. As our understanding of cell physiology and pathological conditions advances, the need for more sophisticated technology and applications continues to push the development of integrated solutions. For many years, BioLegend has been at the front of this development, the most recent example being our Universal TotalSeq™ cocktails to enable multiomic protein analysis by sequencing. Here, we combine several of our cutting-edge reagents for a deep characterization of peptide antigen-induced immune responses. We describe and compare the activation, expansion, and specific T cell response against SARS-CoV-2 spike protein, myelin basic protein, and melanocyte-A immunodominant peptides. T helper subset characterization was achieved with LEGENDplex™ soluble analyte analysis, intracellular and extracellular protein analysis with spectral cytometry, and simultaneous single-cell protein and RNA analysis with our TotalSeq™-C Universal Cocktail. All together, these data provide a unique and comprehensive characterization of in vitro induced viral- and self-peptide specific T cells, revealing similarities and differences in the immune response against viral pathogens and autoimmune diseases.