Monday, May 9, 2022 11:15 AM – 12:00 PM ET Exhibit Hall, Workshop Room 1
Exhibitor Workshop: Bio-Rad Lab., Inc.
Use of StarBright Antibodies to Phenotype Novel Knockout Mice
- Michelle Lennartz, Ph.D., Prof., Albany Med. Col.
Knockout mice are valuable tools for studying infection and immune signaling. However, one must ensure that the gene loss does not have unanticipated effects. We used StarBright antibody cocktails to determine the effects of global or myeloid specific deletion of the oncogene protein kinase C epsilon on immune cell composition and myeloid cell receptor expression. A combined lymphoid myeloid panel, consisting of 11 antibodies, provided a complete picture of the cell types and their representation in spleen and blood. Peritoneal and bone marrow macrophages were interrogated for surface expression of Fc and scavenger receptors and the toll-like receptors.